|
KMID : 0368420170600060604
|
|
Journal of Plant Biology
2017 Volume.60 No. 6 p.604 ~ p.611
|
|
|
Domain of Unknown Function 143 is required for the functioning of PEP-associated protein DG238 in the chloroplast
|
|
Wang Menglong
Jiang Lan
Da Qingen
Liu Jun
Feng Dongru
Wang Jinfa
Wang Hong-Bin
Jin Hong-Lei
|
|
|
Abstract
|
|
|
|
Plastid-encoded plastid RNA polymerase (PEP) is essential for chloroplast development and plastid gene expression in Arabidopsis thaliana. However, PEP is a large complex, and many proteins in this complex remain to be identified. We previously reported that Delayed Greening 238 (DG238) interacts with PEP subunit protein FLN1 and may function as a PEP-associated protein and participate in early chloroplast development and PEP-dependent plastid gene expression. DG238 contains Domain of Unknown Function 143 (DUF143), whose function is currently unknown. Here, we found that a deficiency of the DUF143 domain in DG238 affected its localization, which resulted in abnormal interactions with PEP-associated proteins in the chloroplast. Furthermore, DG238 lacking the DUF143 domain or DG238 with only this domain failed to function. Interestingly, the lack of conserved amino acids 193?217 of the DUF143 domain in DG238 also affected its function. In addition to FLN1, DG238 also interacts with other PEP-associated proteins, including FSD2, FSD3, MRL7-L, and MRL7, to regulate plastid gene expression. These results suggest that the DUF143 domain is necessary for the functioning of the PEP-associated protein DG238 in chloroplasts.
|
|
|
KEYWORD
|
|
|
Arabidopsis, Chloroplast, DG238, DUF143, PEP, Plastid gene expression
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|